Journal: BMC Infectious Diseases

Article Title: Leptospirosis in Indonesia: diagnostic challenges associated with atypical clinical manifestations and limited laboratory capacity

doi: 10.1186/s12879-020-4903-5

Figure Lengend Snippet: Characteristics of cases clinically misdiagnosed as leptospirosis

Article Snippet: For this study, the case definition was adapted as follows: Probable Leptospirosis is a clinically suspected leptospirosis case with a high titer (≥1600) of ELISA IgM antibodies by both Serion and PanBio assays; Confirmed Leptospirosis is a clinically suspected leptospirosis case with a positive qPCR result from plasma or buffy coat and/or a four-fold increase or sero-conversion in the convalescent specimen of ELISA IgM antibodies by Serion and/or PanBio assays.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Colorectal Carcinoma Affected Patients Are Significantly Poor Responders Against the Oncogenic JC Polyomavirus

doi: 10.3389/fimmu.2021.632129

Figure Lengend Snippet: Prevalence of serum immunoglobulin G antibodies reacting to JCPyV viral capsid protein 1 (VP1) mimotopes in indirect ELISA.

Article Snippet: Indeed, an indirect enzyme-linked immunosorbent assay (ELISA) with JCPyV VP1 mimotopes was used.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Colorectal Carcinoma Affected Patients Are Significantly Poor Responders Against the Oncogenic JC Polyomavirus

doi: 10.3389/fimmu.2021.632129

Figure Lengend Snippet: Serologic profile of human serum antibody reactivity to JCPyV viral capsid protein 1 (VP1) mimotopes. Immunologic data are from patients affected by colorectal carcinoma (CRC) and healthy subjects (HS). Results are shown as values of optical density (OD) readings at λ 405 nm for serum samples diluted 1:20 and analyzed by indirect ELISA. Each scatter plot represents the dispersion of individual OD values to a mean level, defined by the line inside the scatter with standard error of the mean (SEM) for each group patients/subjects evaluated. The mean OD for VP1 K (A) , VP1 N (B) and VP1 (K+N) (C) in CRC sera are statistically lower than that observed in HS (****p < 0.0001). Dotted line indicates the cut-off value.

Article Snippet: Indeed, an indirect enzyme-linked immunosorbent assay (ELISA) with JCPyV VP1 mimotopes was used.

Techniques: Indirect ELISA, Dispersion

Journal: Frontiers in Immunology

Article Title: Colorectal Carcinoma Affected Patients Are Significantly Poor Responders Against the Oncogenic JC Polyomavirus

doi: 10.3389/fimmu.2021.632129

Figure Lengend Snippet: Prevalence of serum immunoglobulin G antibodies against JCPyV assessed by haemagglutination inhibition (HAI) assay.

Article Snippet: Indeed, an indirect enzyme-linked immunosorbent assay (ELISA) with JCPyV VP1 mimotopes was used.

Techniques: Inhibition